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摘要晚期糖基化终产物（AGEs）在人体内的蓄积是引起许多慢性疾病如动脉粥样硬化、糖尿病等的重要原因之一。肉桂被发现具有显著的抗氧化活性，研究证明肉桂提取物含有丰富的多酚物质，因此肉桂提取物具有预防心血管疾病、增强人体免疫力和抗癌等作用。本实验拟在食品加工体系中，通过添加天然肉桂提取物来降低食品内的AGEs含量。建立牛血清蛋白（BSA）-葡萄糖的AGEs模拟生成体系，利用荧光检测法测定总AGEs含量，再通过SDS-聚丙烯酰胺凝胶电泳法和western blot（蛋白印迹法）进一步分析。结果表明肉桂提取物对AGEs具有良好的抑制效果。47500
Accumulation of advanced glycation end products (AGEs)in vivo is associated with atherosclerosis ,diabetes, etc. Cinnamon has been found to have significant anti-oxidant activity. The study prove that cinnamon extract containing a large number of phlorotannins. Therefore, cinnamon bark extract have the prevention of cardiovascular disease, enhance immunity and anti-cancer effects. This study aimed at food processing system, by adding natural cinnamon extract to reduce AGEs content of food within. Establish bovine serum albumin (BSA) - glucose analog AGEs generation system, determination of total AGEs content using fluorescence detection method, then by SDS- polyacrylamide gel electrophoresis and western blot (Western blot) for further analysis. The results showed that cinnamon extract have good inhibitory effect on AGEs.
毕业论文关键词：肉桂提取物；晚期糖基化终产物（AGEs）；多酚物质；抑制作用
Keyword: Cinnamon bark extract; Advanced glycation end products (AGEs) ; Phlorotannins; Inhibition
目 录
引言·······················································5
1. 试验材料和仪器·············································6
1.1试验材料················································6
1.2试验试剂················································6
1.3试验仪器················································6
2. 试验方法 ··················································6