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摘要：肉桂精油作为一种新型杀菌剂，因其天然、低毒和较强的抑菌活性成为取代化学杀菌剂的研究热点。微胶囊技术的引入，是改善纯精油商品化过程中遇到瓶颈期的有效手段。本课题构建层层自组肉桂精油微胶囊剂型，测定了粒径，Zeta-电位等表征，并测定了对Escherichia coli和Staphylococcus aureus两种菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值，同时通过直接接触方式研究不同层数自组微胶囊剂型对上述两种菌的抑制效果，观察不同层数自组剂型体内体外抑菌效果的差异，从而为开发精油防腐剂提供理论基础。主要实验结果如下：肉桂精油精油作为芯材制备的微胶囊剂型对Penicillium expansum和Penicillium italicum均具有一定的抑制效果；不同层数的层层自组微胶囊剂型的Zeta-电位和粒径两个指标之间无明显差异；通过测定不同层数自组的肉桂精油微胶囊剂型对E. coli和S.aureus两种菌的MIC和MBC看出，两种微胶囊剂型对E. coli的抑制效果优于其对S. aureus的抑制效果；根据发病率和病斑直径比较抑菌效果可以看出，第五层层层自组溶液在处理P. expansum和P.italicum后的最初三天，抑菌效果相对较好。24187
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毕业论文关键词：肉桂精油、层层自组装、微胶囊、抑菌特性
Formula Design of layer by layer self-assembly microencansules of Cinnamon oil and its simple exploration of bacterial inhibition
Abstract: Cinnamon oil as a new fungicide become research hot spot of chemical fungicides due to its natural, low toxicity and strong antibacterial activity. Microcapsule technology is a good method to improve the bottleneck period of pure essential oils in the commercialization process. The task of constructing the LBL group of cinnamon essential oil microcapsule preparation, determination of the particle size, Zeta- potential characterization, minimum inhibitory concentration (MIC) on E. coli and S.aureus two strain was measured and minimum bactericidal concentration (MBC) value, at the same time through direct contact of different layers self-organizing micro capsule on the above two kinds of bacteria inhibition effect, to observe the difference between different layers self-organizing formulation in vivo antibacterial effect in vitro. Thus providing a theoretical basis for the development of oil. The main results are as follows: Micro capsule cinnamon essential oil as core material prepared had certain inhibition effect on P. expansum and P. italicum. The different layers of the LBL group between micro Zeta- potential and particle size of the capsule two indexes had no significant difference. in MIC and MBC tests. The inhibition effect of different layers of cinnamon essential oil microcapsule against E. coli was much better than S. aureus. According to the comparison of antibacterial activity of incidence and lesion diameter, the fifth layers treated on P. expansum and P. italicum showed better antifungal activity after three days.
Keywords: cinnamon oil；layer by layer self-assembly；microcapsules；antibacterial properties
目 录
1引言 1
1.1 肉桂精油的简介 1
1.1.1 提取肉桂精油的工艺 1
1.1.2 肉桂精油的功效与作用 1
1.1.3 肉桂精油的抑菌性 2
1.2 层层自组装 2
1.2.1 层层自组技术的应用 2
1.3 微囊和微胶囊配方 3
1.3.1 微胶囊和微囊的概述 3
1.3.2 微胶囊和微囊的制法 4
1.4 肉桂精油层层自组微胶囊研究进程 4
1.4.1 制备微胶囊的材料 4
1.4.2 肉桂精油层层自组微胶囊作用 4
1.5 本课题的研究目的和意义 5
2实验材料和方法 6
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