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摘要 目的：以C57BL/6J小鼠为动物模型，研究紫薯提取物对小鼠的抗疲劳与抗氧化作用。方法：选取12周龄健康雌性小鼠20只，适应性喂养1周后，以每组体重相似性为标准分为4组，对照组、紫薯提取物低剂量处理组（100㎎/㎏）、中剂量组（200㎎/㎏）及高剂量组（400㎎/㎏），喂养处理4周后，对小鼠进行一次性负重力竭游泳（按小鼠3％体重负重），建立小鼠疲劳模型，并记录力竭游泳时间。游泳结束后，处死小鼠，分离血清和部分肝脏及腿部肌肉组织，分别测定血尿素氮（BUN），丙二醛（MDA），肝糖原及肌糖原含量。结果：相比对照组，紫薯提取物中剂量和高剂量处理小鼠的力竭游泳时间显著延长，抗疲劳能力提高；血清MDA含量显著减少，抗氧化能力增强；同时，三种剂量紫薯提取物处理组小鼠血清BUN含量显著减少，说明小鼠非蛋白质能量代谢能力增强。结论：一定剂量的紫薯提取物能够显著提高小鼠的抗疲劳能力。49877
毕业论文关键词：小鼠；紫薯提取物；抗疲劳；抗氧化；丙二醛
Study on effects of purple sweet potato extract on anti-fatigue and anti-oxidation in mice
Abstract Purpose: The anti-fatigue and anti-oxidation effects of purple sweet potato extract in C57BL / 6J mice were investigated. Methods: 20 healthy female mice of 12 weeks old were selected as animal materials. After one week of adaptive treatment, they were pided into four subgroups by weight: the control group, low dose treatment group of purple potato extract (100 mg/kg), middle dose group (200 mg/kg) and high dose group (400 mg/kg). After four-weeks feeding, one exhaustion swimming test was performed for all mice and the swimming times were recorded.
Then, all mice were sacrificed and the blood, liver and muscle tissues were sampled to measure the content of blood urea nitrogen (BUN), malondialdehyde (MDA), liver glycogen and muscle glycogen, respectively. The results showed that, compared with the control group, middle and high dose treatment group of purple potato extract significantly prolonged swimming time, reduced serum MDA content, and all dose purple potato extract-treated groups markedly reduced the serum BUN content of mice. In concussion, a certain dose of purple potato extract can significantly improve the anti-fatigue capacity of mice.
Key words: mice; purple potato extract; anti-fatigue resistance; antioxidant; MDA
目 录
摘 要 1
Abstract 1
引言 2
1材料与方法 2
1.1材料与试剂 2
1.2仪器与设备 3
1.3小鼠处理与指标测定 3
1.3.1小鼠喂养及分组 3
1.3.2小鼠血清和组织的分离与提取 3
1.3.3小鼠体重测定 3
1.3.4小鼠竭力游泳时间测定 4
1.3.5 血尿素氮BUN含量测定 4
1.3.6丙二醛MDA含量测定 4
1.3.7肝糖原和肌糖原含量测定 5
1.4数据分析 6
2结果与分析 6
2.1紫薯提取物对小鼠体重的影响 6
2.2紫薯提取物对小鼠竭力游泳时间的影响 7
2.3紫薯提取物对小鼠体内血尿素氮含量的影响