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摘要：解淀粉芽袍杆菌(Bacillus amyloliquefaciens)SQR9是本实验室从黄瓜根际分离的一株促生菌株，对黄瓜专化型病原菌尖袍镰刀菌有很强的拮杭功能。在盆栽和大田试验中，该菌株表现出良好的促生和土传病害防控效果，其与腐熟堆肥制成的微生物有机肥在农业生产中已得到广泛应用。生物依赖色氨酸合成IAA的几种途径中，都有其特定的中间产物，根据这些特定中间产物分别命名为吲哚丙酮酸途径、色胺途径、吲哚乙酰胺途径、吲哚乙腈途径途径。本实验通过在解淀粉芽袍杆菌SQR9和突变体ΔysnE中添加各种途径的中间产物，分析SQR9和ΔysnE的发酵液的IAA和这7种关键组分，从而寻找基因ysnE参与IAA合成的途径。经过实验分析得出结论：吲哚丙酮酸（IPA）途径和色胺（TAM）途径可能是SQR9依赖色氨酸合成IAA最主要的两个途径。基因ysnE可能分别参与IAA合成途径中的吲哚丙酮酸途径和色胺途径，而且是在吲哚丙酮酸到吲哚乙醛之间及色胺到吲哚乙醛之间发挥功能。基因ysnE没有参与吲哚乙酰胺（IAM）途径和吲哚乙腈（IAN）途径。35417
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毕业论文关键词：解淀粉芽孢杆菌；突变体ΔysnE；IAA合成途径；产IAA能力
the study of Capacity of IAA synthesis from Bacillus amyloliquefaciens SQR9 and mutant in different IAA synthesis intermediates the indoles
Student majoring in Agricultural Resource and Environment WangMingliang
Tutor ZhangRuifu
Abstract：Bacillus amyloliquefaciens SQR9, isolated from the rhizosphere of cucumber, can control Fusarium wilt of cucumber. The strain was proved to be an outstanding plant growth-promoting rhizobacteria (PGPR) strain by its ability to inhibit the growth of Fusarium oxysporum f. sp. cucumerinum J. H. Owen (FOC) and decrease the incidence of Fusarium wilt in field experiments, and a bioorganic fertilizer made of organic fertilizer fermented with B. amyloliquefaciens SQR9 is widely used in agricultural production.Biological dependence on tryptophan synthesis of IAA in several ways, has its specific intermediate, according to the specific intermediate respectively named IPA, TAM, IAM,and IAN.This experiment through the solution of starch bud robe coli SQR9 and mutant ΔysnE, various ways of intermediate product is added to the analysis of SQR9 and ΔysnE fermented liquid of IAA and the seven key components, the ysnE to search for genes involved in IAA synthetic way. The conclusion of the experiment is:IPA and TAM approach may be the two main pathways of the SQR9 dependence on tryptophan synthesis IAA. Gene ysnE may participate in IAA respectively synthetic ways of indole pyruvate way and tryptamine way, and it is between indole pyruvate and indole acetaldehyde and serotonin function between indole acetaldehyde. Gene ysnE is not involved in IAM and IAN.
Key words: Bacillus amyloliquefaciens；mutantΔysnE；IAA synthesis pathway；Capacity of IAA synthesis
目 录
摘要 1
关键词 1
Abstract 1
Key words 2
引言 2
1 材料与方法 3
1.1 材料 3
1.2 方法 3
1.2.1 发酵液制备 3
1.2.2 发酵液样品处理 3
1.2.3 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中IAA定量分析 3
1.2.4 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中TOL和ILA定量分析 5
1.2.5 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中TAM定量分析 5
1.2.6 不同吲哚衍生物处理的SQR9和ΔysnE发酵液中IAN和IAM定量分析 5
2 结果与分析 5