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摘要本文采用固相萃取-高效液相色谱法（SPE-HPLC）建立了水体中的马来酸氨氯地平与阿替洛尔这2种阳离子药物及个人护理用品（PPCPs）的检测方法。用甲醇- KH2PO4作为流动相，在温度：30℃、进样量：15ul的条件下，研究了马来酸氨氯地平与阿替洛尔的日内精密度、日间精密度、加标回收率、最低检测限。其结果显示，马来酸氨氯地平的日内精密度范围在4.16%-12.24%，日间精密度在2.95%-11.88%，加标回收率在90%-110%，最低检测限为5.2ug/L；阿替洛尔日内精密度范围在4.72%-14.23%，日间精密度在2.88%-12.22%，加标回收率在95%-112%，最低检测限为6.9ug/L。分别采用C18、Poly-Sery HLB、WATER OASIS HLB对2种药物进行了富集提取。其结果显示，马来酸氨氯地平采用Poly-Sery HLB、100%甲醇洗脱，得到最高回收率为71.3%。阿替洛尔采用WATER OASIS HLB、90%甲醇洗脱，得到的最高回收率为88.4%。59869
关键词：固相萃取；高效液相色谱法；马来酸氨氯地平；阿替洛尔；分析检测
毕业设计说明书（毕业论文）外文摘要
Title Analysis methods for the PPCPs pollutants in water
Abstract
In this paper, a solid-phase extraction-high performance liquid chromatography (SPE-HPLC) method has been developed for the determination of maleate amlodipine and atenolol these two kinds of cationic pharmaceuticals and personal care products (PPCPs) in water. In the temperature: 30 ℃、the injection volume: 15ul conditions, making methanol - KH2PO4 as the mobile phase todetect maleate amlodipine and atenolol for the intraday precision, the interday precision, the recovery and the minimum detection limit. The results showed that the intraday precision of maleate amlodipine ranges from 4.16% to12.24%, interday precision ranges from 2.95% to 11.88%,the recovery ranges from 90% to 110% ,the minimum detection limit is 5.2ug/L ; the intraday precision of atenolol ranges from 4.72% to 14.23%, interday precision ranges from 2.88% to 12.22%, and the recovery ranges from 95% to 112%, the minimum detection limit is 6.9ug/L. C18、Poly-Sery HLB、Water Oasis HLB were used respectively to enrich and extract for two kinds of drugs. The results showed that maleate amlodipine uses Poly-Sery HLB, 100% methanol as eluent and obtain a maximum recovery rate of 71.3%; atenolol uses Water Oasis HLB, 90% methanol as eluent and obtain a maximum recovery rate of 88.4%.
Keyword: solid phase extraction; high performance liquid chromatography; maleate amlodipine; atenolol; determination
1 绪论 1
1.1 药物与个人护理用品（PPCPs） 1
1.2 PPCPs水样分析检测 2
1.2.1取样与预处理 2
1.2.2 富集提取 3
1.2.3 净化 3
1.2.4 检测 3
1.3 固相萃取 4
1.4 高效液相色谱技术 4
1.5 选题目的与意义 5
2 实验部分 6
2.1 实验试剂与仪器 6
2.2 流动相及母液的配置 7
2.2.1 流动相的配置 7
2.2.2 马来酸氨氯地平母液的配置 7
2.2.3 阿替洛尔平母液的配置