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摘要 在溶剂萃取过程中，随着萃余液循环使用，以夹带、溶解等方式流失到萃余液中的有机物会进入到生物浸出环境，从而对微生物浸矿过程产生影响。本文通过模拟 LIX984N浓度为 5%和 10%的有机物污染过程，考察细菌、培养基不同的受染方式下 A.t菌的生长、在黄铜矿表面的吸附规律以及浸矿过程。结果显示，在有机物污染下A.t菌的生长受到抑制，随着 LIX984N浓度的增加，抑制作用加强，A.t 菌在有机物不同污染方式下活性降低的趋势为（活性由大到小排序）：纯培养>只有细菌受染>只有培养基受染>培养基与细菌均受染。在吸附过程中，受到有机物不同方式污染时A.t 菌在黄铜矿表面的吸附率均高于细菌与培养基均未受污染的吸附率，随着有机物浓度的升高吸附率升高，而达到吸附平衡的时间也相应的减少，其中，只是培养基受污染时吸附率最高为 72.18%，达到吸附平衡的时间也最短为 11min，细菌与培养基均未受染的吸附率最低为 43.36%，达到吸附平衡的时间也最长为 20min。 36039
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毕业论文关键词：溶剂萃取；氧化硫硫杆菌；有机污染；吸附；生物浸铜
Abstract In the solvent extraction process, with the raffinate recycled to entrainment and dissolution, etc，is lost to the raffinate organic encounter into bioleaching environment to have an impact on microbial leaching process. In this article, Through simulation LIX984N concentration of 5% and 10% of the organic phase fouling process, study the bacteria and medium that is under different pollute mode Acidothiobacillus thiooxidans growing, attach regular pattern chalcopyrite surface and leaching process. The results showed that the growth in the organic phase polluted Acidothiobacillus thiooxidans was inhibited with increasing concentrations LIX984N inhibition strengthen, In the organic phase in different pollution ways activity may decrease Acidothiobacillus thiooxidans is (active in descending order): pure culture > Only bacteria polluted > only medium polluted > Both medium and bacteria are polluted. In the attachment process, a different mode of pollution of the organic phase when Acidothiobacillus -thiooxidans attachment rate chalcopyrite surface more than bacteria and medium both polluted, among them, only medium polluted when attachment rate highest as 72.18%, Achieve the shortest time attachment equilibrium as 11min, bacteria and media have not been polluted when the lowest rate of attachment as 43.36%, attachment equilibrium longest time 20min.
KeyWords：Acidothiobacillus thiooxidans; organic phase pollution; attach; bioleaching copper
目录
1绪论.1
1.1研究背景.1
1.2嗜酸氧化硫硫杆菌.2
1.3生物浸出机制.2
1.3.1直接作用.2
1.3.2间接作用.3
1.3.3直—间接接触作用机制.3
1.4铜萃取有机物对生物浸出的影响.5
1.5细菌在黄铜矿表面的吸附.5
1.6研究的目的、意义和内容.6
1.6.1研究的目的和意义.6
1.6.2研究内容.6
2实验内容与方法.7
2.1实验材料.7
2.1.1矿样.7
2.1.2菌种.7
2.1.3萃取剂.7
2.1.4稀释剂.7
2.1.5实验试剂.7
2.1.6实验仪器.8
2.2实验方法.8
2.2.1矿样制备.8
2.2.2细菌培养与驯化.8
2.2.3溶剂萃取过程模拟.8
2.2.4有机物不同污染方式下A.t菌的培养.9
2.2.5有机物不同污染方式下A.t菌的吸附.9
2.2.6有机物不同污染方式下黄铜矿的生物浸出10
2.3性能表征10
2.3.1细胞浓度测定10
2.3.2pH与电位的测定11
2.3.3溶液中Fe2+
含量滴定方法.11
3结果与讨论13
3.1有机物对A.t菌生长的影响13
3.1.15%LIX984N有机物对A.t菌生长的影响.13