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摘要本课题主要研究了树脂对漆酶的吸附固定作用，固定化漆酶的酶活，以及固定化漆酶对农药毒死蜱的酶促降解作用。为了更加明确吸附和降解作用，对漆酶的酶学性质做了相关研究，结果表明：漆酶在25℃、35℃、45℃下保存5天后，相对酶活仍能保持60%以上，特别是在25℃下，酶活几乎没有损失；漆酶在酸性条件下，酶活相对较高，当pH为5.0时，漆酶酶活最高；漆酶的最佳吸附树脂为D113，在酶浓度为15%，吸附4小时后吸附率可达30%；固定化漆酶的最佳反应条件与漆酶相似，pH为5.0时酶活最高；固定化漆酶保存一周后无明显酶活损失；在毒死蜱的酶促降解方面，上述固定化漆酶可降解毒死蜱，降解率约35%。研究结果表明该酶具有较好的热稳定性和pH稳定性,对不同环境均具有较好的耐受力,有良好的应用前景。10394
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关键词 漆酶 固定化 降解 毒死蜱毕业设计说明书（论文）外文摘要
Title ： Immobilized laccase enzymatic degradation of the pesticide chlorpyrifos
Abstract
This thesis mainly studies the adsorption and immobilization of resin on the laccase, the immobilized laccase activity, as well as the immobilized laccase on the enzymatic degradation of chlorpyrifos.In order to make the adsorption and degradation clearer,related researches were performed on laccase enzymatic properties,and the results showed that: reserved in 25 ℃, 35 ℃, 45 ℃ for 5 days, the enzyme can still maintain more than 60% of relative activity , especially little loss at 25 ℃; Enzyme activity of laccase is relatively high in acidic conditions,while it presents the highest activity at pH 5; The best adsorption resin belongs to D113,the adsorption rate reaches 30% after exposure 4 hours if the enzyme concentration is 15%;The optimum reaction condition of immobilized laccase and laccase were similar,that's to say the highest activity is at pH 5;There exsits no significant loss of enzyme activity after being stored a week;The immobilized laccase can degrade chlorpyrifos, with degradation rate of about 35%.The results show that such enzyme has better thermal stability and pH stability in different environments, thus a promising application prospect.
Keywords ：Laccase Immobilized degradation Chlorpyrifos
目 录
第一章 前 言 1
1.1 毒死蜱 1
1.1.1 毒死蜱的应用概况及存在问题 1
1.1.2 毒死蜱的降解机制 2
1.1.3 毒死蜱微生物降解的研究进展 2
1.2 漆酶 3
1.2.1 漆酶的来源 3
1.2.2 漆酶的结构及理化性质 3
1.2.3 漆酶催化氧化反应机理 4
1.2.3.2 催化氧化反应机理 4
1.2.4 漆酶在环境保护方面的应用 5
1.2.5 漆酶的固定化研究及应用进展 6
1.3 本论文的研究思路和内容 6
第二章 漆酶酶学性质的研究 7
2.1 实验部分 7
2.1.1主要试剂和仪器 7
2.1.2漆酶酶活的测定方法 7
2.1.3 酶活影响因素 8
2.2 结果与讨论 8
2.2.1漆酶最适反应温度 9
2.2.2漆酶热稳定性 9
2.2.3 漆酶最适反应pH值 11
2.2.4 漆酶pH值稳定性 12
2.2.5 不同浓度的缓冲溶液对漆酶酶活的影响 12
2.3 本章小结 13