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摘 要：为了探讨腐胺提高大豆幼苗抗盐性的机理，本实验以“郑豆30”为材料，采用沙培法，研究了在盐胁迫下，腐胺对大豆幼苗叶片渗调物质含量的影响。结果表明：大豆幼苗在盐胁迫下，电解质渗透率、可溶性蛋白含量、脯氨酸（Pro）、可溶性总糖含量均显著升高，叶绿素含量也有升高的趋势，而相对干物质增长率显著下降。但经过腐胺（Put）处理后，大豆幼苗内电解质渗透率、可溶性蛋白含量、脯氨酸（Pro）、可溶性总糖含量的升高均受到了抑制，叶绿素含量的影响不明显；而相对干物质增长率的下降有所缓解。这说明腐胺（Put）可以缓解盐胁迫对大豆幼苗造成的伤害, 增强其抵抗能力，从而延缓盐胁迫下大豆幼苗的衰老，促进大豆幼苗的生长。9959
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关键词：腐胺；盐胁迫；大豆幼苗；渗调物质
Effects of Putrescine on Osmoregulation Substance of Soybean Seedlings Leaves under Salt Stress
Abstract: In order to explore the mechanism that putrescine improves soybean seedlings resistance to salt stress, takeing "Zhengdou 30" as material, we studied the effects of putrescine on osmoregulation substance of soybean seedlings under salt stress. The results are as follows: the electrolyte leakage, the contents of soluble protein, proline (Pro), soluble total sugar of soybean seedling leaves were significantly increased, chlorophyll content also has a tendency to rise，however, the relative dry matter growth rate decreased significantly under salt stress. After the treatment with putrescine (Put), the electrolyte leakage, the contents of soluble protein, proline (Pro), soluble total sugar of soybean seedling leaves were significantly inhibited, the influence of putrescine on chlorophyll content is not obvious, but putrescine could improve the relative growth rate of dry matter of soybean seedlings under salt stress. The results show that putrescine (Put) can alleviate the damage of salt stress on soybean seedlings, enhance their ability to salt resistance, delay the aging of soybean seedlings under salt stress, and promote the growth of soybean seedlings.
Key Words: Putrescine; Salt stress; Soybean seedlings; Osmoregulation substance
目录
摘 要： 1
关键词： 1
引言 2
1.材料与方法 2
1.1材料 3
1.2试剂 3
1.3材料培养与处理 3
1.4 测定内容与方法 3
1.4.1幼苗相对干物质增长速率 3
1.4.2相对电解质渗透率测定 4
1.4.3叶绿素含量测定 4
1.4.4可溶性蛋白含量测定 4
1.4.5可溶性糖含量测定 5
1.4.6游离脯氨酸含量测定 6
2.结果与分析 7
2.1 Put对盐胁迫下大豆幼苗相对干物质增长速率的影响 7
2.2 Put对盐胁迫下大豆幼苗叶片相对电解质渗透率的影响 7
2.3 Put对盐胁迫下大豆幼苗叶片叶绿素总含量的影响 8
2.4 Put对盐胁迫下大豆幼苗叶片可溶性蛋白含量的影响 8
2.5 Put对盐胁迫下大豆幼苗叶片脯氨酸含量的影响 9
2.6 Put对盐胁迫下大豆幼苗叶片可溶性总糖含量的影响 9
3.结论与讨论 10
参考文献 12
致谢 14
腐胺对盐胁迫下大豆幼苗叶片渗调物质含量的影响引言
大豆属于豆科大豆属大豆种，一年生草本，原产于中国。全国各地均有栽培，以东北最随著名，广泛栽培与世界各地[1]。2008年，我国大豆产量位于世界第四，仅次于美国、巴西、阿根廷，年产量上百万吨[2]。现在世界上45%的大豆田地和55%的产量来自美国。大豆成为最重要的粮食作物之一，同时大豆是植物蛋白和食用油的主要来源，也是世界上产量最多的油料作物。因此，大豆的功能举足轻重，鉴于大豆对人们生活生产的重要作用，研究大豆的抗逆性具有重要的意义。