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摘要：本论文探讨了用95%乙醇作为溶剂采用索氏提取法、95%乙醇超声波提取法和95%乙醇溶剂浸提法这三种方法对植物桑椹进行提取。测其抗氧化性，并对粗提物的吸湿性，失水性进行测定，将得到的粗提物作为天然有效成分加入保湿膏霜中制成保湿霜。通过对配制工艺的探讨、产品的质量检测和在保湿霜中的配伍性进行研究，结果表明：（1）采用紫外分光光度法测试，经过羟自由基清除率试验以及DPPH自由基清除率试验可知95%乙醇提取液的体外抗氧化活性较强。（2）对桑椹粗提物进行吸水性和失水性测试，均得到较好的结果。（3）将得到的粗提物加到保湿膏霜里，进行40℃耐热检测、-5℃耐寒检测、耐热耐寒综合检测，有无刺激性等性能检测后，目测样品无分层或析出，无颜色变化，无气变化。证明桑椹粗提物可加入保湿膏霜中并具有良好的稳定性。说明植物桑椹在化妆品的抗衰老和保湿方面具有一定的应用前景。7142
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关键词： 桑椹提取；紫外分光光度法；抗氧化；保湿
Mulberry extract the effective components and effect evaluation of oxidation and moisturizing effect
Abstract: This paper discusses the Chinese herbal medicine plant mulberry extract with the method of 95% ethanol Soxhlet extraction、95% ethanol ultrasonic extraction and 95% ethanol solvent-extraction method. Measure the oxidation resistance of the extracting, and the crude extracting hygroscopic, water were lost, and will get crude extracting as effective natural moisturizing cream in composition to join made moisturizing cream. Through the discussion of configuation craft, product quality inspection and moisturizing cream in the compatibility of research, the results show that:(1) with ultraviolet spectrophotometry, after Oxidation resistance experiments, 95% ethanol mulberry extraction of in vitro antioxidant activity is quite strong.(2) the mulberry get crude extracting water and water loss on the test, get good results.(3) getting crude extracting added to the moisturizing cream, to 40 ℃and-5 ℃, heat and cold test, heat tolerance to cold comprehensive detection test, have excitant or not, and the performance after test. Find visual sample no layering or separation, no color change, no odour change. Proof of crude extracting mulberry can join the moisturizing lotion and has good stability. Chinese herbal medicine in cosmetics that identify mulberry anti-aging and moisturizing have a prospect of application.
Key words: mulberry extracted; Ultraviolet spectrophotometry; antioxidant; moisturizing
目录
1 前言..1
1.1 天然化妆品的发展1
1.2桑椹国内外的研究现状概述2
1.2.1 桑椹的主要成分..2
1.2.2桑椹的药理作用3
1.3本课题的研究意义和目的5
1.3.1医药工业5
1.3.2食品工业6
2 实验原理与方法6
2.1 实验原料与仪器设备.6
2.1.1 实验原料.6
2.1.2 实验仪器与设备.7
2.2 实验步骤..8
2.2.1 桑椹粗提物的提取..8
2.2.2 旋转蒸发仪9
2.2.3 冷冻干燥装置..10
2.2.4 桑椹粗提物的抗氧化性测定.11
2.2.5 桑椹粗提物吸湿性的测定12
2.2.6 保湿霜基霜的配制.13
2.2.7 保湿霜的理化性能的测试方法..15
3 实验结果与讨论17
3.1提取率的计算与研究..17
3.2 桑椹粗提物的羟自由基清除率的研究18
3.3 桑椹粗提物的DPPH自由基清除率的研究.19
3.4 对吸水性及失水性的结果讨论.20
3.4.1 吸水性的结果讨论.20
3.4.2 失水性的结果讨论.22