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2.5 14个牛品种的聚类分析 18
3 讨论 19
3.1微卫星位点选取问题 19
3.2遗传距离和亲缘关系 19
3.3前景展望 20
4 结论 21
参考文献 21
致谢 22
图清单
图序号 图名称 页码
图1-1 主要仪器设备 8
Figure 1-1 Apparatus in present study 8
图1-2 微卫星PCR扩增体系 12
Figure 1-2 Microsatellite PCR amplification system 12
图1-3 微卫星PCR扩增程序 12
Figure 1-3 The Procotol of microsatellite PCR Reaction 12
图2-1 1.5%的琼脂糖电泳检测提取的DNA 14
Figure 2-1 1.5% agarose electrophoresis of genomic DNA 14
图2-2 微卫星位点CSSM66PCR扩增产物琼脂糖凝胶电泳检测 14
Figure 2-2 PCR amplification product detection of Microsatellite loci CSSM66 by agarose gel electrophoresis 14
图2-3 HEL1位点电泳扩增图 14
Figure 2-3 Electrophoresis patterns of representive PCR products of HEL1 Loci 14
图2-4 CSSM66位点电泳扩增图 15
Figure 2-4 Electrophoresis patterns of representive PCR products of CSSM66 Loci 15
图2-5 ETH10位点电泳扩增图 15
Figure 2-5 Electrophoresis patterns of representive PCR products of ETH10 Loci 15
图2-6 ILSTS06位点电泳扩增图 15
Figure 2-6 Electrophoresis patterns of representive PCR products of ILSTS06 Loci 15
图2-7 MM8位点电泳扩增图 15
Figure 2-7 Electrophoresis patterns of representive PCR products of MM8 Loci 15
图2-8 MM12位点电泳扩增图 15
Figure 2-8 Electrophoresis patterns of representive PCR products of MM12 Loci 15
图2-9 INRA005位点电泳扩增图 16
Figure 2-9 Electrophoresis patterns of representive PCR products of INRA005 Loci