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摘要：目的：确定西红花球茎、幼叶愈伤组织诱导的适宜激素配比，为西红花的组织培养、快繁等研究奠定基础和科学依据。方法：以西红花球茎部位为材料，以MS为基本培养基，以激动素(KT)、吲哚丁酸（IBA）、萘乙酸（NAA）作为单因素变量，确定诱导西红花球茎愈伤组织诱导的适宜条件；以MS+0.5 mg•L-16-BA为基本培养基，添加不同浓度的2,4-D作为变量，确定诱导西红花幼叶愈伤组织形成的条件。结果：在仅含有KT的培养基中，诱导球茎形成愈伤组织效果最好；且浓度为0.2 mg•L-1KT的诱导率最高，诱导率为71.00%。在激素组合中，诱导西红花幼叶产生愈伤组织的最佳激素组合是MS+0.5 mg•L-16-BA+1.2 mg•L-1 2,4-D。诱导率为67.00%。结论：在单种植物生长调节剂的处理下，KT诱导西红花球茎形成愈伤组织的诱导率高。在幼叶诱导形成愈伤组织中，以MS+0.5 mg•L-16-BA+1.2 mg•L-1 2,4-D 培养基效果最好，诱导率最高。30502
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毕业论文关键词：西红花；组织培养；愈伤组织；植物生长调节剂
Study on the tissue culture of Crocus sativus
[Abstract] Objective: To study the most suitable hormone and the concentration of callus induced with the young leafs and corms of Crocus sativus, laying the foundation and scientific basis for the breeding of saffron cultivation. Method: The corms of Crocus sativus cultured in the medium that was MS supplemented with single plant growth regulator, as kinetin (KT), indole butyric acid (IBA) and 1-Naphthylacetic acid (NAA), to determine the appropriate conditions of hormone induced callus of saffron. The young leafs of Crocus sativus cultured in the medium which was added 0.5 mg•L-16-BA of MS supplemented with different concentration of 2,4-D, to determine the appropriate conditions of hormone induced callus of saffron. Result: In the study of induced callus with corms of Crocus sativus, it was the best medium of KT, and the induction rate was 71.00% at the concentration of 0.2 mg•L-1. In the study of hormone combination , saffron Leaves Callus Induced by hormone combination was the best MS+0.5 mg•L-16-BA+1.2 mg•L-1 2,4-D. The induction rate was 67.00%. Conclusion: In the treatment of single plant growth regulator induced callus, the medium with KT induced the highest induction rate. In young leafs callus induction, the medium with MS+0.5 mg•L-16-BA+1.2 mg•L-12,4-D had the best effect and the highest induction rate.
Key words: Crocus sativus L.; tissue culture; callus; plant growth regulators
目 录
摘要 1
关键词 1
Abstract. 1
Key words 1
1 材料与方法 2
1.1 试验材料 2
1.2 试验方法 2
1.2.1 外植体灭菌方法 3
1.2.2 培养基的配制 3
1.3 测定项目 3
1.4 数据处理 3
2 结果与分析 3
2.1 不同外植体对西红花诱导产生愈伤组织的影响 3
2.2 不同激素对西红花球茎诱导产生愈伤组织的影响 4
2.3 激素组合对诱导西红花幼叶产生愈伤组织的影响 4
3 讨论 5
3.1 番红花生长规律与外植体选取 5
3.2 植物激素及不同配比对愈伤组织诱导影响 6
3.2.1 不同激素对西红花球茎诱导产生愈伤组织的影响 6
3.2.2 激素组合对西红花幼叶诱导产生愈伤组织的影响 6
3.3 附加物对西红花诱导形成愈伤组织的影响 6