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摘要：为了深层次评估抗虫、抗除草剂复合性状转Cry1C*/Bar基因水稻T1c-19向杂草稻基因漂移的潜在风险，在单种无虫压和自然虫压下，对T1c-19与三种杂草稻（广东茂名、湖南益阳和江苏泰州）的F3（以杂草稻为母本的杂交后代为F3+，以T1C-19为母本的杂交后代为RF3+）的适合度进行了研究。试验结果表明，无论是在无虫压还是自然虫压下，F3MM+和RF3MM+的总适合度显著高于T1c-19，其它杂交后代的总适合度也达到与T1c-19相当的水平。与亲本杂草稻相比，在无虫压下，各F3与相应亲本杂草稻的总适合度相似。在自然虫压下，尽管在单株干生物量以及结实率上部分F3具有明显优势，但供试F3的总适合度与相应亲本杂草稻相似。比较自然虫压和无虫压下的适合度，具有抗性基因的T1C-19和F3以及杂草稻在自然虫压下总适合度与无虫压下无显著差异。由此可以看出，在本试验条件下，供试F3+和RF3+与亲本T1c-19和杂草稻相比，具有相似或更高的适合度。因此务必防范该转基因水稻和杂草稻之间的基因漂移。26676
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毕业论文关键词：T1c-19；适合度；复合性状转基因水稻；杂草稻
The fitness of F3 between transgenic rice T1C-19 with stacked genes and weedy rice in pure plot
Abstract： In order to deeply assess the potential risks of gene flow from transgenic rice T1c-19 with stacked Cry1C*/Bar genes to weedy rice, the fitness of F3 between transgenic rice T1C-19 and three weedy rice (Guangdong Maoming, Hunan Yiyang and Jiangsu Taizhou，F3+ denoted T1c-19 as paternal plants, RF3+ denoted T1c-19 as maternal plants) were studied in pure plot under natural insect pressure and no insect pressure. The results demonstrated that the composite fitness of F3MM+ and RF3MM+ were significantly greater than that of T1c-19 under natural insect pressure and no insect pressure. The other F3 had similar composite fitness to T1c-19. Under insect pressure and no insect pressure, six kinds of F3+ and RF3+ was as fit as their respective weedy rice, although some of F3 had greater biomass per plant and seed set than their weedy rice. Compared composite fitness of T1c-19, F3 and weedy rice under insect and no insect pressure, it was found all experimental materials displayed similar fitness. From above results, gene flow between T1c-19 and weedy rice should be avoided because both F3+ and RF3+ had similar or greater composite fitness compared to T1c-19 or their respective weedy rice.
Key words: T1c-19; Fitness; Stacked transgenic rice;Weedy rice
目 录
摘要3
关键词3
Abstract3
Key words3
引言3
1材料与方法4
1.1试验材料 4
1.2试验设计 5
1.2.1选择压设置5
1.2.2靶标虫压的调查和计算方法5
1.2.3适合度指标测量5
1.2.4数据统计5
2结果复分析6
2.1靶标虫压的调查6
2.2 T1c-19与F3+和RF3+在不同虫压下的适合度比较6
2.2.1无虫压下，T1c-19与F3+和RF3+的适合度比较6
2.2.2自然虫压下，T1c-19与F3+和RF3+的适合度比较7
2.3 WR与F3+和RF3+在不同虫压下的适合度比较 8
2.3.1 无虫压下，WR与F3+和RF3+的适合度比较8
2.3.2 自然虫压下，WR与F3+和RF3+的适合度比较9
2.4 亲本稻T1C-19和杂草稻及子代F3+和RF3+在无虫压和自然虫压中的适合度比较10
3讨论 11
致谢12
参考文献12
复合性状转基因水稻T1c-19与杂草稻的F3在单种条件下的适合度
引言：
随着转基因技术的迅速发展和转基因作物大规模的商业化种植，转基因作物的环境安全问题逐渐受到关注[1-2]。其中最值得关注的就是抗性基因通过花粉逃逸到野生近缘杂草中，产生携带抗性基因的杂交后代，由此带来的潜在生态风险[3]。在评估转基因作物的基因漂移风险时，除了评估转基因作物和近缘杂草能否发生初始杂交之外，携带抗性基因的杂交或回交后代在不同生态条件下的适合度也是非常重要的评价内容[4-5]。适合度是用来衡量个体或种群在不同环境下的适应程度，即特定基因型个体在同种生物群体中生存和繁殖后代的能力[6]。