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摘要：以黄瓜品种‘津优4号’为材料，采用营养液栽培，研究外源葡萄糖（Glu）对75 mM NaCl胁迫下黄瓜幼苗生长、光合作用以及碳水化合物的影响，探讨葡萄糖缓解盐胁迫下黄瓜幼苗的生理机制。结果表明，叶面喷施100 mmol∙L-1 Glu显著提高了盐胁迫下黄瓜幼苗株高、茎粗、地上部干鲜重，缓解了盐胁迫诱导的生长抑制作用；盐胁迫施用Glu显著提高黄瓜叶片气孔导度和光合色素含量；此外，施用外源Glu增加盐胁迫下黄瓜叶片蔗糖、果糖和总糖含量，而显著降低了淀粉的含量。这些结果表明，外源葡萄糖通过调节碳水化合物代谢，提高了盐胁迫下黄瓜幼苗光合作用，促进植株生长，进而增强盐胁迫下黄瓜植株的抗性。¬25681
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毕业论文关键词：黄瓜幼苗；葡萄糖；盐胁迫；碳水化合物
Effects of exogenous glucose on growth and carbohydrate metabolism of cucumber seedlings under salt stress
Abstract：The cucumber cultivar 'Jinyou No. 4' as material, a solution culture experiment was conducted to study the effect of exogenous glucose (Glu) of 75 mM in Cucumber Seedlings under NaCl stress effects on growth, photosynthesis and carbohydrate, on glucose to alleviate the physiological mechanism of Cucumber Seedlings under salt stress. The results showed that foliar application of 100 mmol.L-1 Glu significantly improved the cucumber seedlings under Salt Stress on plant height, stem diameter, shoot fresh and dry weight, alleviate the inhibition of salt stress induced growth; salt stress significantly decreased stomatal conductance and leaf photosynthetic pigment, application of Glu enhanced salt stressed cucumber seedlings in addition, the photosynthesis; promote cucumber leaf sucrose, fructose and total sugar content accumulation of exogenous Glu under salt stress, but decreased the content of starch in 7 days. These results indicated that exogenous glucose could enhance the photosynthesis of Cucumber Seedlings under salt stress and promote plant growth by regulating carbohydrate metabolism.
Key words: cucumber seedlings; Glucose; salt stress;carbohydrate
目 录
摘要1
关键词1
Abstract1
Key words1
引言 1
1 材料与方法2
1.1材料生长 2
1.2试验处理 2
1.3测定指标及方法2
1.4数据处理3
2 结果与分析3 2.1 外源Glu对NaCl胁迫下黄瓜幼苗生长的影响3
2.2 外源Glu对NaCl胁迫下黄瓜幼苗光合作用的影响4
2.2.1外源Glu对NaCl胁迫下黄瓜幼苗光合参数的影响4
2.2.2外源Glu对盐胁迫下黄瓜幼苗光合色素含量的影响5
2.3 外源Glu对NaCl胁迫下黄瓜幼苗碳水化合物含量的影响5
2.4 外源Glu对NaCl胁迫下黄瓜幼苗碳水化合物代谢关键酶编码基因表达的影响6
3 讨论 8
4 结论8
致谢9
参考文献9
图1 外源Glu对NaCl胁迫下黄瓜幼苗地上部形态的影响3图2 外源Glu对NaCl胁迫下黄瓜幼苗光合参数的影响4
图3 外源Glu对NaCl胁迫下黄瓜幼苗碳水化合物含量的影响6
图4 外源Glu对NaCl胁迫下黄瓜幼苗碳水化合物代谢关键酶编码基因表达的影响7
表1 外源Glu对NaCl胁迫下黄瓜幼苗生长的影响4
表2 外源Glu对NaCl胁迫下黄瓜幼苗叶片光合色素含量的影响5
外源葡萄糖对盐胁迫下黄瓜幼苗生长和碳水化合物代谢的影响
引言
近年来，我国设施蔬菜栽培发展非常迅速，栽培产量和面积都位居世界前列[1]。由于复种指数大，水肥灌溉频率大以及设施内处于封闭的环境条件，使设施内部温度高、蒸发量大，从而造成设施土壤盐分积聚越来越严重。目前，土壤次生盐渍化已成为国内外设施栽培中普遍存在的难题[2]。研究表明，盐胁迫下植株根吸收表面积减小、膜通透性增加以及叶片数减少，加剧了水分失衡[3]，严重阻碍了设施蔬菜的可持续、优质和高效发展[4]。