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摘要：土壤盐渍化会影响作物质量和产量，并会限制农业发展。本文立足于已有的研究报道，利用转基因技术结合激光共聚焦显微镜观察，初步研究了参与囊泡运输的RAB5小G蛋白与水稻抗盐机制之间存在的联系。结果表明，RAB5b作为囊泡运输过程中的调节因子，在盐胁迫下，其组成型激活态的过表达可以文持Na+/H+反向转运子（NHX1）定位于液泡膜上；而NHX1可以将存在于细胞质内的高浓度Na+泵入液泡内存储起来，即区室化，从而增强植株的耐盐性、减少了高浓度盐离子对细胞器和酶类的伤害。本文为通过遗传手段培育耐盐作物提供了新的思路及理论依据。25552
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毕业论文关键词：植物耐盐机制；囊泡运输；RAB蛋白；区室化；Na+/H+反向转运子
Functions of plant-specific RAB5 homologous genes in salt tolerance in rice
Abstract: Soil salinization affects crop quality and yield, and limits agricultural development. This paper, based on the existing research reports, using the transgenic means combined with laser scanning confocal microscopy, preliminarily discusses the relationship between RAB5, a small G protein functioned at vesicular transporting, and rice salt tolerance mechanism. The results showed that RAB5b, as a regulatory factor in the process of vesicular transportation, under salt stress, its constitutively active state overexpression could enhance the placement of NHX1 on the vacuolar membrane. NHX1 could be used to pumped Na+, which located at cytoplasm in high concentration, into the vacuoles. That process is also called compartmentation. In this way, the effect of salt ions’ damage to organelles and enzymes will mitigate, and plant salt tolerance will enhance. This article provides a new idea and theoretical basis for cultivating salt-tolerant crops through genetic means.
Key words: plant salt tolerance mechanism; vesicular transportation; RAB protein; compartmentation; NHX1
目 录
摘要1
关键词1
Abstract1
Key words1
引 言 1
1 材料与方法 3
1.1 植物材料 3
1.2 试剂及各种化学药品 3
1.3 实验仪器 3
1.4 方法 3
1.4.1 拟南芥室内培养 3
1.4.2 拟南芥盐处理 3
1.4.3 Na+/Ca2+染色方法（用于激光共聚焦显微镜观察） 4
1.4.4 农杆菌的转化 4
1.4.5 农杆菌介导的拟南芥转化 4
1.4.6 拟南芥杂交及后代筛选方法 4
2 结果与分析 4
2.1 RAB5bCA过表达增强了Na+的区室化 5
2.1.1 RAB5bCA过表达对植株表型的影响 5
2.1.2 RAB5bCA过表达对植株体内Na+分布的影响 5
2.1.3 RAB5bCA过表达对植株中Ca2+分布的影响 6
2.2 RAB5bCA过表达对NHX1蛋白质亚细胞定位的影响 7
2.2.1 在无盐胁迫情况下，RAB5bCA过表达对NHX1的运输无影响 7
2.2.2 盐胁迫下RAB5bCA过表达能够文持NHX1的正确膜定位 7
3 讨论 8
3.1 RAB5bCA通过囊泡运输过程调控植株耐盐性 8
3.2 本研究的意义及创新性 9
致谢 9
参考文献: 9
植物特有的RAB5同源基因在水稻抗盐中的机理研究