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摘 要：以野生大豆“ZS”作为实验材料，采用室内沙培，探究不同浓度（0、5、10、15mg/L）1,3,4-噻二唑衍生物（N-[5-(4-氯苯基)-1,3,4-噻二唑-2-基]-N'-4-硝基苯甲酰基脲）对除草混剂胁迫下野生大豆幼苗生长的影响。结果表明：（1）除草混剂胁迫下野生大豆幼苗植株变矮，鲜重降低，叶片内叶绿素、可溶性蛋白、可溶性糖含量明显降低，而叶片内MDA与游离Pro含量水平显著上升（P<0.01）。（2）适当浓度N-[5-(4-氯苯基)-1,3,4-噻二唑-2-基]-N '-4-硝基苯甲酰基脲处理增加了除草混剂胁迫下野生大豆幼苗的株高、鲜重，且叶绿素含量、可溶性蛋白、游离脯氨酸和可溶性糖含量均显著高于胁迫下的野生大豆幼苗，而MDA的含量则显著降低（P<0.01）。该试验表明适当浓度1,3,4-噻二唑衍生物（N-[5-(4-氯苯基)-1,3,4-噻二唑-2-基]-N'-4-硝基苯甲酰基脲）的处理能有效缓解和修复除草混剂对野生大豆幼苗的伤害，具有显著促进野生大豆生长的作用，其中10mg/L TNU处理效果最好。10836
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关键词：野生大豆；除草混剂；噻二唑衍生物；生理特性
Effect of herbicide mixed preparations on Wild Soybean Seedlings and the Research of 1,3,4-Thiadiazole Derivative on its Role
Abstract：Using wild soybeans “ZS” as experiment material, effects of the different concentration (0, 5, 10 and 15mg/L) of N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) on the growth of wild soybean seedling under herbicide mixed preparations stress was studied by sand culture. The results showed as follow: （1）under herbicide mixed preparations stress, the height of seedling and fresh weight were significantly decreased, while the content of chlorophyll, soluble proteins and soluble sugar were significantly decreased than those of control. The contents of proline and MDA were significantly increased(P<0.01).(2) Exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole-2-yl]-N'- 4-Nitrobenzoyl Urea (TNU) application on the leaf significantly increased the height of seedling and fresh weight, and the contents of chlorophyll, soluble proteins, soluble proline were significantly increased(P<0.01). The content of malonaldehyde (produce of membrane lipid peroxidation) was significantly decreased(P<0.01). The results showed that exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) could effectively mitigate the harmful effects from herbicide mixed preparations stress on plants, and promote the growth of wild soybeans, and the effect of 10mg/L TNU was optimal.
Key Words：1,3,4-thiadiazole; herbicide mixed preparations; Soybean; Physiological characteristics
目 录
摘要 1
ABSTRACT 1
引言 2
1材料与方法 2
1.1试验材料 2
1.2试验方法 3
1.2.1材料处理 3
1.2.2 测定项目与方法 3
1.3数据分析 4
2结果与分析 4
2.1 TNU对除草混剂胁迫下野生大豆幼苗苗高的影响 4
2.2 TNU对除草混剂胁迫下野生大豆幼苗苗鲜重的影响 4
2.3 TNU对除草混剂胁迫下野生大豆幼苗叶片内叶绿素含量的影响 5
2.4 TNU对除草混剂胁迫下野生大豆幼苗叶片内可溶性蛋白含量的影响 6
2.5 TNU对除草混剂胁迫下野生大豆幼苗叶片内脯氨酸含量的影响 7
2.6 TNU对除草混剂胁迫下野生大豆幼苗叶片内可溶性糖含量的影响 8
2.7 TNU对除草混剂胁迫下野生大豆幼苗叶片内MDA含量的影响 9
3讨论 10