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摘要 介孔二氧化硅（MSNs）作为一类新型的药物载体在药物缓控释领域的应用方兴未艾。本课题采用模板诱导和自组装方法合成MCM-41型介孔二氧化硅纳米颗粒，并通过聚乙二醇（PEG）的表面修饰，制备出一类具有高亲水性、高孔隙率、高比表面积的MSNs修饰微球。采用氮吸附（BET）、扫描电镜（SEM）、透射电镜（TEM）、粒径分析等手段表征并对比了聚乙二醇（PEG）修饰前后的MSNs基础理化性能。在此基础上，选用阿霉素作为模型药物，包埋于介孔材料中，对比了PEG修饰前后MSNs中阿霉素的装载量、包封率及释放行为。选用乳腺癌细胞MCF-7为模型癌细胞，人血清蛋白（HAS）为模型蛋白，考察了两种材料对细胞的生物安全性及对蛋白的吸附情况，并将两种材料所对应的载药微球与MCF-7进行共培养，探究两种载药系统对癌细胞的灭杀情况。结果表明，较之MSNs，PEG修饰后的MSNs微球具有更小粒径（~100nm）、更大孔容和更高的比表面积等特点，并具有较高的载药率和包封率。由于PEG的修饰，载药MSNs微球具有更加明显的缓释特性，且其药物作用时间更长，灭杀癌细胞效果更好。36099
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毕业论文关键词： 介孔二氧化硅；PEG表面修饰；药物缓控释系统；生物安全性；抗癌
Synthesis and characterization of mesoporous silica nanoparticles for drug delivery Abstract As a new type of drug carriers，Mesoporous silica nanoparticles (MSNs) is in the ascendant in the field of drug control and release. In this article, both the template induced and self-assembling method was employed to synthesize the MCM-41 type of mesoporous silica nanoparticles. With the co-polymerization with polyethylene glycol (PEG), MSNs with wormhole pore structure were obtained with high hydrophilic, high porosity and specific surface area. The modification of PEG to the MSNs was characterized by nitrogen adsorption (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and particle size analysis. Besides, the influence of PEG modification to the drug loading content, the encapsulation efficiency and the release behavior of the MSNs were also analyzed with the doxorubicin as a model drug. To compare the biosecurity and protein adsorption of the two kinds of materials, the human breast cancer cell MCF-7 and the human serum albumin (HAS) were selected as the model cancer cells and the model protein, respectively. On the other hand, to investigate the anti-cancer effect of these two drug delivery systems, the materials were co-cultured with MCF-7 cells and determined the quantity of cells by means of Microplate Reader. The results show that, compared with unmodified MSNs, MSNs-PEG were spherical morphology with smaller size (100 nm), larger pore volume and higher specific surface area. And with the PEG modification, MSNs has higher drug loading rate and entrapment rate. Owing to the modification of PEG, the drug loaded MSNs microspheres have more evident of controlled-release properties and better anticancer properties.
KeyWords： Mesoporous Silica Nanoparticles; PEG modification; Sustained and Controlled Release Drug Delivery; Biosecurity; Anticancer
目录
1文献综述..1
1.1引言.1
1.2纳米靶向载药系统.1
1.2.1纳米靶向载药系统的定义1
1.2.2纳米靶向载药系统的分类2
1.2.3纳米靶向载药系统的优势3
1.3缓控释载药系统.3
1.3.1药物的缓释与控释3
1.3.2缓控释载药系统制剂的研究进展3
1.4介孔二氧化硅纳米颗粒.4
1.4.1MSNs材料的合成机理.5
1.4.2MSN制备工艺的研究进展..6
1.5基于MSNs表面功能化的药物控释7
1.5.1酸性基团修饰MSNs的药物控释.8
1.5.2碱性基团修饰MSNs的药物控释.8
1.5.3中性基团修饰MSNs的药物控释.8
1.6本课题研究的目的和意义.9