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摘 要：本论文主要利用NCBI,DNAman,Expasy等相关软件，采用生物信息学方法对人类、袋獾、地山雀、斑马鱼、锦龟、热带爪蟾、黄金蟒、扬子鳄和腔棘鱼九种含有TXNDC17基因的物种进行了亲缘关系分析，并对人类的TXNDC17基因所编码蛋白质的氨基酸组成、理化性质、亚细胞定位、跨膜区、信号肽、二级结构和三级结构及功能域等多方面进行了分析和预测。结果表明，TXNDC17基因在进化过程中不是很保守，九种生物的基因相似性仅达到15.92%。基因进化过程中主要分化为两大支，一类分支为硬骨鱼类、两栖类和腔棘鱼类；另一分支为哺乳动物（包括胎盘动物和有袋动物）、鸟类、海蜥蜴和鳄目。对人类TXNDC17基因编码的蛋白分析得出，该蛋白共含有124个氨基酸，并存在TRP14-like保守结构域，其蛋白属于Thiordoxin-like超家族，具有多个不同的磷酸化位点，不存在跨膜区，以及N端序列不存在任何信号肽序列，说明该蛋白质定位在细胞质基质，且不属于膜蛋白或分泌蛋白。9244
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关键词：TXNDC17；生物信息学；进化树；蛋白分析
The Evolution Analysis of TXNDC17 Gene
Abstract: The genetic relationship of nine creatures were analyzed which are Homo sapiens, Sarcophilus harrisii, Pseudopodoces humilis, Danio rerio, Chrysemys picta, Xenopus tropicalis, Python bivittatus, Alligator sinensis and Latimeria chalumnae containing TXNDC17 gene and the protein encoded by TXNDC17Gene in human was analyzed and forecasted from the aspects of protein amino acid composition, physical and chemical properties, the subcellular localization, across the membrane area, signal peptide, secondary structure, tertiary structure and functional domains and so on through bioinformatics methods by softwares, such as NCBI, DNAman Expasy and so on. The results show that TXNDC17 gene in the process of evolution is not very conservative and the genetic similarity of nine creatures is only 15.92%. TXNDC17 gene in the process of evolution is pided into two branch, one are bony fish, amphibians and cavity spines fish and another are mammals (including animal placenta and marsupials), birds, lizards and sea crocodilians. The results analyzing the protein encoded by TXNDC17gene in human show that the protein has 124 amino acids and exists TRP14-like conservative domain structure which belongs to Thiordoxin-like super family and it has different phosphorylation sites and does not exist Transmembrane structure and signal peptide sequence in N-terminal sequence which indicate the protein is located in cytoplasmic matrix and does not belong to the membrane proteins or secreted proteins.
Keywords: TXNDC17; Bioinformatics; The evolutionary tree; Protein analysis
目 录
摘要 1
引言 2
1. 材料与方法 2
1.1 物种的获取 2
1.2 基因进化分析 3
1.3 分析方法与工具 3
2. 结果与分析 4
2.1 基因结构与定位分析 4
2.2 九种生物TXNDC17基因的比较和同源进化树的构建。 6
2.3 人类TXNDC17基因所编码蛋白的生物信息学结果与分析 8
3. 讨论与结论 13
参考文献 14
致谢 16
TXNDC17基因的进化分析引言
硫氧还蛋白域17(TXNDC17)基因是一个蛋白编码基因，用来编码与电子载体活动和二硫化物蛋白还原酶的活动相关的蛋白[1]。硫氧还蛋白是细胞中普遍存在的低分子量蛋白质，为生物体所必需。硫氧还蛋白、硫氧还蛋白还原酶和烟酰胺腺嘌呤二核苷磷酸组成硫氧还蛋白系统，具有调节细胞的氧化还原状态 [2]，抗凋亡及促进细胞增殖等功能[3]。硫氧还蛋白能改变植物自交不亲和性状，具有抗旱、耐热、抗氧化胁迫和对抗逆基因的表达进行调控的功能[4]。