摘 要:马铃薯采后贮藏过程中易发生褐变和品质劣变,抗坏血酸(AsA)和CaCl2是公认高度安全的食品与果蔬保鲜剂。本试验通过正交设计实验方案,研究了不同浓度AsA与 CaCl2复合处理对马铃薯在室温贮藏条件下果肉抗氧化酶系统4个指标的变化情况。结果表明:与对照组(CK)及其他3组处理方案相比,Treat 2(0.2% AsA +0.4% CaCl2,浸泡10 min)在贮藏期间能显著维持马铃薯较高的SOD、POD、CAT抗氧化酶活性,清除了自由基,减少MDA含量的积累,因此能够有效延缓马铃薯采后品质劣变和细胞衰老进程。 65503
毕业论文关键词:马铃薯,抗坏血酸,氯化钙,抗氧化酶,保鲜
Effect of Ascorbic Acid Combined with Calcium Chloride Treatments on Antioxidant Enzyme System of Potatoes(Solanum tuberosum L.)
Abstract: Postharvest potatoes are easy to cause the browning and quality deterioration during storage. Ascorbic acid (AsA) and CaCl2 are recognized as efficient and safe preservatives of foods, fruits and vegetables. This experiment, designed by Orthogonal experimental design, researched the changes of four indicators of antioxidant enzyme system of potatoes with the different concentration of AsA and CaCl2 under room temperature storage conditions. The results show that treat 2 (0.2% AsA + 0.4% CaCl2, soak 10 min),compared to the control (CK) and other three treatment scheme groups, can significantly maintain the higher activities of SOD, POD and CAT enzymes of potatoes during storage, remove the free radicals and reduce the accumulation of MDA content. So it can effectively delay the quality deterioration and aging process of postharvest potatoes.
Keywords: Potato (Solanum tuberosum L.), Ascorbic acid, Calcium chloride, Antioxidant enzymes, Preservation
目 录
前言 5
1 材料与方法 5
1.1 试验材料 5
1.2 试验设计与处理 5
1.3 检测指标与方法 6
1.3.1 超氧化物歧化酶(SOD)活性的测定 5
1.3.2 过氧化物活性酶(POD)活性的测定 6
1.3.3 过氧化氢活性酶(CAT)活性的测定 6
1.3.4 丙二醛(MDA)含量的测定 6
1.4 实验数据处理 7
2 结果与分析 7
2.1 不同浓度AsA结合CaCl2处理对马铃薯SOD活性的影响 7
2.2 不同浓度AsA结合CaCl2处理对马铃薯CAT活性的影响 7
2.3 不同浓度AsA结合CaCl2处理对马铃薯POD活性的影响 8
2.4 不同浓度AsA结合CaCl2处理对马铃薯MDA含量的影响 9
3 讨论 9
结 论 11
参考文献 12
致 谢 13
前言
马铃薯原产于南美洲,现已成为全球第四大重要的菜粮兼用经济作物,营养丰富,具有健胃和解毒消肿等药用价值。马铃薯采后在室温下贮藏,很容易出现褐变、青变及发芽等品质劣变现象,产生龙葵素等有毒物质,易引起食物中毒[1]。因此在室温贮藏条件下探寻安全、有效、经济的果蔬保鲜剂仍是解决马铃薯采后保鲜问题的关键技术之一。论文网