摘要:目的:探究银杏叶提取物(EGB)对小鼠的抗疲劳作用。方法:将12只雄性小鼠平均分成对照组和处理组,以一定浓度的EGB水溶液(64.8mg/day/Kg)对处理组喂食,4周后,对所有小鼠进行一次性负重力竭游泳实验,记录游泳时间,并解剖取血液,肝组织和肌肉组织分别测血尿素氮(BUN),丙二醛(MDA),肝糖原和肌糖原含量。结果:相比对照组,处理组游泳时间明显延长,丙二醛(MDA)含量显著降低,肌糖原含量显著提高,说明EGB能有效改善小鼠抗疲劳能力,提高小鼠肌糖原的合成以增加肌糖原含量,增强小鼠抗氧化能力,削弱丙二醛等对细胞的毒害作用。结论:一定剂量的银杏叶提取物(EGB)对小鼠具有抗疲劳作用。50336
毕业论文关键词:银杏叶提取物(EGB);抗疲劳;抗氧化;小鼠
Study on anti-fatigue effect of Ginkgo biloba extract on mice
Abstract: To investigate anti-fatigue effect of Ginkgo biloba extract (GBE) on mice, 12 male mice were pided into control and GBE-treated (64.8mg/day/kg) after one week adaptive treatment. After four-weeks of treatment, one exhaustion swimming test was performed for all mice and the swimming times were recorded. Then, all mice were sacrificed and the blood, liver and muscle tissues were sampled to measure the content of blood urea nitrogen (BUN), malondialdehyde (MDA), liver glycogen and muscle glycogen, respectively. The results showed that, compared with the control group, the swimming time of EGB-treated group significantly prolonged, the content of malondialdehyde (MDA) decreased and the content of muscle glycogen increased significantly. In conclusion, some dose of EGB supplements can improve effectively the anti-fatigue effect on mice.
Keywords: Ginkgo biloba extract (EGB); anti-fatigue; antioxidant; mice
目 录
摘 要 1
Abstract 1
引言 1
1材料与方法 2
1.1实验动物 2
1.2材料与试剂 2
1.3仪器与设备 3
1.4小鼠处理与指标测定 3
1.4.1小鼠的饲养与分组 3
1.4.2小鼠血清和组织的分离与提取 3
1.4.3小鼠采食量与体重测定 3
1.4.4小鼠竭力游泳时间测定 4
1.4.5血尿素氮BUN含量测定 4
1.4.6丙二醛MDA含量测定 4
1.4.7肝糖原和肌糖原含量测定 5
1.5 数据分析 6
2 结果与分析6
2.1 银杏叶提取物对小鼠采食量和体质量的影响 6
2.2 银杏叶提取物对小鼠竭力游泳时间的影响.8
2.3 银杏叶提取物对小鼠体内血尿素氮含量的影响 8
2.4 银杏叶提取物对小鼠体内丙二醛含量的影响 9
2.5 银杏叶提取物对小鼠体内肝糖原和肌糖原含量的影响 9
3讨论 10
参考文献 12
致谢 14
银杏叶提取物对小鼠抗疲劳作用的研究引言
疲劳是指机体运动本身所引起的不能持续其功能在特定水平或不能维持预定的运动强度的生理现象[1]。据世界卫生组织调查,早在20世纪末,全球约35%以上的人处于疲劳状态,中年男性人群疲劳状态达60.7%[2],近70%的中年人处于疲劳状态[3]。在生活节奏日益加快的现代社会,部分人群特别是农村外出务工人员盲目地过度运动现象凸显,这样会对机体产生很大的损伤,甚至会带来器质性病变。因此,通过食品或药物干预来提高机体的抗疲劳能力和工作效率意义十分重大。