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摘要:表面增强拉曼（SERS）光谱技术广泛应用于多个领域，如生命科学、材料科学、催化化学与环境化学等研究领域。而利用基于纳米粒子的SERS光谱技术来研究生物活性分子在不同纳米材料界面的行为研究则结合了SERS光谱技术和纳米粒子的诸多优点，大大推进了SERS光谱技术在涉及生物分子研究领域应用的广度与深度。因此，本论文制备了稳定性好、灵敏度高的SERS基底材料，用于探测多巴胺及巯基取代物在不同纳米材料表面的SERS活性。该论文以多巴胺、巯基取代多巴胺为目标分子，通过紫外光谱及SERS光谱技术研究了生物活性分子在金属纳米颗粒界面的光谱行为。紫外光谱和SERS光谱研究结果表明，巯基取代多巴胺分子与金和银纳米颗粒之间的结合力更强。该论文为进一步研究与纳米材料作用的生物分子的结构与性质及分子与金属表面之间的结合状态与作用机理奠定了基础，且对于深入理解生物分子结构与其功能间的关系具有十分重要的意义。7099
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关键词: 表面增强拉曼光谱；紫外光谱；金属纳米颗粒；多巴胺
The spectroscopic behavior study of active biomolecules on different nanomaterials interface
Abstract: Surface Enhanced Raman Scattering (SERS) spectroscopic technique has been applied to a wide range of fields, such as life science, material science, catalytic chemistry, environmental chemistry etc. However, the study of the behavior of bioactive molecules in the interface of different nanometer material through SERS spectroscopic technique based on nano particle makes use of the advantages of both SERS spectroscopic technique and the nano particle, thus expand the breadth and depth of application of the SERS spectroscopic technique in the research fields concerned with biomolecule. This paper prepares SERS substrate material that is of good stability and high sensitivity to explore the SERS activity of dopamine and sulfydryl substituendum on surfaces of different kinds of nanometer material. This paper takes dopamine and sulfydry (the replacement of dopamine) as the target molecule, studies the spectral behavior of bioactive molecule in the interface of metal nanoparticles with the help of ultraviolet spectrum and SERS spectroscopic technique. The results imply that using sulfyryl to replace dopamine generates a stronger binding force with golden and silver nanoparticle. This paper paves the way for the further study of the structure and properties of biomolecule when interacting with nanometer materials, and the binding state and mechanism of action between molecule and metal surface. In addition, this paper is of great importance in better understanding the relation between bimolecular structure and its function.
Keywords：Surface Enhanced Raman Scattering；Ultraviolet spectrum；metal nanoparicles；Dopamine
目录
1. 文献综述
1.1 拉曼光谱与表面增强拉曼散射(SERS)光谱
1.1.1 拉曼散射的基本原理及特点
1.1.2 SERS现象及机理
1.1.3 拉曼光谱及SERS光谱的应用概况
1.2 纳米材料简介
1.2.1 纳米材料的基本效应
1.2.2 SERS基底的制备状况
1.2.3 金属纳米颗粒的重要性
1.2.4 银纳米材料和金纳米材料的制备方法
1.3 紫外光谱简介
1.3.1 分子对光的选择性吸收
1.3.2 朗伯-比尔定律
1.4 多巴胺介绍
1.5 课题的目的和意义
2. 实验部分
2.1 试剂和仪器
2.1.1 试剂
2.1.2 仪器
2.2 试剂合成
2.2.1 银胶的合成