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摘要：本试验采用基质栽培的方法，选用‘长绿’苦瓜和‘津优35号’黄瓜为试验材料。幼苗长至两叶一心时采用劈接法进行嫁接。幼苗长至三叶一心时，进行四组处理：自根常温（Cs-28℃），自根高温（Cs-42℃），异根常温（Mc-28℃），异根高温（Mc-42℃）。结果表明：高温胁迫下，嫁接黄瓜幼苗的株高、茎粗、苗干鲜重都受到了不同程度的影响，但利用苦瓜嫁接黄瓜幼苗的生长指标优于黄瓜自嫁接。通过研究苦瓜嫁接黄瓜幼苗与黄瓜自嫁接幼苗之间的差异蛋白点发现，表现差异的蛋白点主要包括光合作用相关蛋白、能量代谢相关蛋白、防御响应相关蛋白。因而苦瓜嫁接黄瓜缓解高温胁迫主要是通过调控植物体内的光合作用、能量和代谢、防御响应的过程，减轻高温胁迫对黄瓜幼苗造成的损伤。37289
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毕业论文关键词：黄瓜；嫁接；高温胁迫
Effects of high temperature stress on physiological growth of grafted cucumber
Abstract：In this study, the ‘long green’ bitter gourd and ‘Jinyou 35’ cucumber were used as experimental materials.When the seedlings grow to two leaves, they are grafted by splitting.When the seedlings were as long as three leaves, they were treated in four groups: self root temperature (Cs-28℃), self root temperature (Cs-42℃), different root temperature(Mc-28℃), and different root temperature (Mc-42℃).The results showed that the plant height, stem diameter, seedling dry weight and fresh weight of grafted cucumber seedlings were affected to some extent under high temperature stress, but the growth index of cucumber seedlings grafted with bitter gourd was better than that of cucumber self grafting.Through the study of bitter gourd grafted cucumber seedlings and cucumber seedlings grafted from the difference between proteins found differentially expressed protein spots mainly including photosynthesis related proteins, proteins related to energy metabolism, defense response related proteins.Therefore, bitter gourd grafted cucumber alleviate the high temperature stress, mainly through the regulation of plant photosynthesis, energy and metabolism, defense response process, and alleviate the damage caused by high temperature stress on cucumber seedlings.
Key words: Cucumber；Grafting；High-temperature stress
目 录
摘要1
关键词1
Abstract1
Key words1
引言 1
1 材料与方法2
1.1 植物材料与幼苗培养 2
1.2 试验处理 2
1.3 测定方法2
1.3.1 生长指标测定2
1.3.2 叶片蛋白质的提取2
1.3.3 双向凝胶电泳2
1.3.4 染色和图像处理分析2
1.3.5 蛋白点质谱鉴定和数据搜索3
2 结果分析 3
2.1 高温胁迫对嫁接黄瓜生长的影响3
2.2 高温胁迫对嫁接黄瓜蛋白质的影响3
2.2.1 差异蛋白对光合作用的影响4
2.2.2 差异蛋白对能量和代谢的影响7
2.2.3 差异蛋白对防御响应的影响8
2.2.4 差异蛋白对蛋白质和核酸合成的影响10
2.2.5 未知功能蛋白11
3 小结11
致谢12
参考文献12
图1 高温胁迫对嫁接黄瓜幼苗叶片蛋白质表达的影响3
表1 高温胁迫下嫁接黄瓜株高、茎粗、叶面积、干重、鲜重、壮苗指数的影响3
表2 差异蛋白中与光合作用相关的蛋白点4
表3 差异蛋白中与能量和代谢相关的蛋白点8
表4 差异蛋白中与防御响应相关的蛋白点9
表5 差异蛋白中与蛋白质和核酸合成相关的蛋白点10
表6 未知功能蛋白11
高温胁迫对嫁接黄瓜生理生长影响
高温胁迫给露地和设施蔬菜栽培带来了严重的影响。我国许多地区夏季以及春夏、夏秋之交时，不管是日光温室还是塑料大棚内，由于强光和高温的叠加效应，棚内温度急剧增长，即使利用通风降温系统，温室内的温度仍然很高，白天气温经常超过黄瓜开花坐果的适宜温度范围[1]，严重影响植株的正常生长。