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摘 要：几丁质酶（EC3.2.1.14)是一类在于植物、动物、微生物中的能降解几丁质的糖苷酶，与植物体内抗真菌性的防御系统有关，是一种重要的病程相关蛋白。本文通过NCBI数据库已登录的小麦（Triticum aestivum AAR11388.1）、高粱（Sorghum bicolor XP-002488863.1)、水稻（Oryza sativa japonica group NP_001050373.2)、蓖麻（Ricinus communis XP_002525743.1)、拟南芥（Ar-abidopsis thaliana XP_00284907.1)、玉米（Zea mays AAT40016.1) 等植物的蛋白序列进行查询下载，分别对其进行理化性质分析、亚细胞定位、跨膜区、磷酸化位点分析、同源比对、构建进化树以及二、三级结构预测等一系列生物信息学分析。结果表明，这些蛋白均存在信号肽序列，均为分泌性蛋白，定位于细胞液中，含有多个不同的磷酸化位点；选择的751类植物的几丁质酶蛋白序列长度和等电点差异不大，这几种蛋白序列均存在5-8个紊乱区，它们都属于球蛋白，且序列中20中常见氨基酸组成接近；751种植物几丁质酶的二级结构均以无规则卷曲为主。通过进化树的构建可知，通过构建进化树得知，这几种植物相似性较高，进化距离较近。这些结果为几丁质酶的进一步深入研究提供了理论基础。35625
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毕业论文关键词：植物；几丁质酶；生物信息学
Bio-informatics Analysis of Plant Chitinase
Abstract:Chitinase (EC3.2.1.14) is a class that plants, animals, microorganisms can degrade chitin (N- acetylglucosamine linear oligomers) glycosidases. Its resistance to fungi and plants related to defense systems, is an important pathogenesis-related proteins. We queryed and download Triticum aestivum （ AAR11388.1）、Sorghum bicolor（ XP-002488863.1)、Oryza sativa japonica group （NP_001050373.2)、Ricinus communis （XP_002525743.1)、Arabidopsis ( XP_00284907.1)、Zea mays (AAT40016.1) protein sequence of the chitinase from the NCBI database. We are analyzed their basic physical and chemical properties, subcellular localization, transmembrane region, homology comparison and phylogenetic tree, as well as secondary and tertiary structure prediction and a series of bioinformatic analysis to understand its structure and function related information. These results indicate that the signal peptide sequences of these proteins are present, are secreted proteins, located in the cytosol, it contains a number of different phosphorylation sites; chitinase protein sequence length and isoelectric selection of six plants point is insignificant, these types of protein sequences are present disorder zones 5-8, they all belong to globulin, and the sequence of 20 amino acids commonly found close; the secondary structure of the six plants of chitinase are no rules curly-based. Rhodopseudomonas palustriss because they had close evolutionary distance. Seen through the evolutionary tree constructed by constructing evolutionary trees that, these types of higher plant similarity, close evolutionary distance. All results provide a theoretical basis for further research.
Keywords: plant; chitinase; Bioinformatics analysis
目 录
摘 要 1
引 言 2
1 材料和方法 3
1.1材料来源 3
1.2 生物信息学分析软件 4
2 结果与分析 5
2.1生物信息学分析 5
2.1.1 基本理化性质分析 5
2.1.2几丁质酶跨膜区预测 7
2.1.3几丁质酶的信号肽分析 8
2.1.4 几丁质酶的亚细胞定位 8
2.1.5几丁质酶磷酸化分析 9
2.2 几种蛋白序列的同源性比对与同源进化树的构建 9
2.3 二级结构、蛋白紊乱区、球蛋白区预测 11
2.3.1蛋白质二级结构预测 11