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摘要： 目的：探究三黄汤对幼龄蚂蟥消化道疾病预防作用的最适浓度。 方法：采用观察不同浓度三黄汤(3.00‰、1.50‰、0.3‰、0.15‰、0.03‰)对消化道免疫低下的蚂蟥生长、存活情况，消化道组织形态结构的变化，以及蚂蟥生长，消化及非特异免疫基因表达量的变化，筛选预防蚂蟥消化道疾病的三黄汤浓度并阐明机制。 结果：与其他组别相比，0.15‰的组效果最显著，存活率和特定生长率明显增高(p<0.05)。0.15‰的组蚂蟥消化道接近于正常蚂蟥消化道。实验组生长激素GH均明显高于对照组(p<0.05)，0.3‰的组消化酶基因表达量显著提高(p<0.05)。SOD、CAT、AKP基因表达量均比对照组高，0.15‰和0.3‰的组显著提高(p<0.05)。 结论：0.15‰三黄汤能够恢复消化道免疫低下幼龄蚂蟥消化道组织结构，改善其非特异性免疫力并促进其生长。31335
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毕业论文关键词：蚂蟥；三黄汤；生长；非特异性免疫酶；消化道
Effects of Sanhuang Decoction on growth and immune of young Whitmania pigra Whitman
Abstract：Objective:To explore the most suitable concentration of Sanhuang Decoction on the prevention of the young w. Pigra digestive tract diseases. Methods: The effects of different concentrations of Sanhuang Decoction on gastrointestinal immune w.pigra survival, growth, changes of histological structure of the digestive tract, and w. pigra superoxide dismutase SOD gene expression, screening concentration of Decoction to prevent w. Pigra digestive tract disease and to elucidate the mechanism. The result, compared with other groups, The effect of 0.15 per 1000 group was the most remarkable, and the survival rate was 34.3% higher than that of the control group, and the specific growth rate was obviously higher (p<0.05). The digestive tract 0.15 1000 w. Pigra group close to the normal w. Pigra digestive tract. SOD gene expression in the experimental group were higher than the control group, the effect of 0.15 per 1000 group was the most remarkable(p<0.05). The conclusion,0.15 per 1000 decoction can restore digestive tract in immunocompromised w.pigra histological structures of digestive tract, improve their immunity and promote its growth.
Key words: Whitmania Pigra Whitman；Sanhuang Decoction；gene to non-specific immunity；digestive tract
目 录
摘要3
关键词3
Abstract3
Key words3
引言（或绪论）3
1材料与方法4
1.1材料 4
1.2方法 4
1.2.1蚂蟥消化道免疫力低下模型的建立4
1.2.2不同浓度三黄汤对蚂蟥生长的影响4
1.2.3样品的石蜡切片制作4
1.2.4样品的生长激素基因表达量的测定 4
1.2.5样品的消化酶基因（淀粉酶、脂肪酶、蛋白酶）表达量的测定4
1.2.6样品的非特异性免疫酶基因（SOD、CAT、AKP）表达量的测定 4
2 结果与分析5
2.1各浓度组的生长数据分析5
2.1.1各浓度组的存活率 5
2.1.2各浓度组平均增重率 5
2.1.3各浓度组特定生长率 5
2.2蚂蟥消化道结构变化分析 6
2.2.1食道的结构变化 6
2.2.2除第6侧盲囊外的嗉囊的结构变化 6
2.2.3第6对侧盲囊的结构变化 7
2.2.4肠部的结构变化 7
2.3 蚂蟥生长、消化酶及非特异性免疫酶基因表达量比较分析 8
2.3.1蚂蟥生长激素基因相对表达量 8
2.3.2蚂蟥消化酶基因相对表达量 8
2.3.3蚂蟥非特异性免疫酶基因相对表达量 8
3讨论8
3.1三黄汤对蚂蟥特定生长率及存活率的影响8
3.2三黄汤对蚂蟥消化道结构变化的分析9
3.3三黄汤对蚂蟥消化酶基因表达量分析 9
3.4三黄汤对蚂蟥非特异性免疫酶基因表达量分析10
3.2小结与展望10
致谢 11
参考文献 12