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摘要：本试验欲通过夏季添加精氨酸，探究精氨酸对高温季节公猪精液抗氧化能力的影响。选取体重、精液品质相似，品种相同的成年种公猪（12-24月龄）20头，在2个月的饲养期中分别饲喂含精氨酸质量分数为0.6% 、0.8%和1.0% 的精制饲料，采集精液并测定精浆中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-pX）和总超氧化物歧化酶（T-SOD）的活性及丙二醛（MDA）含量等抗氧化指标。结果表明：夏季添加精氨酸可提高公猪精液中过氧化氢酶（CAT）活性，其中1.0%组过氧化氢酶（CAT）活性高于对照组，并产生显著差异（P<0.05）。日粮精氨酸水平对谷胱甘肽过氧化物酶（GSH-pX）活性有促进作用，0.8%组和1.0%组与对照组相比较差异显著（P<0.05）。总超氧化物歧化酶（T-SOD）的活性得到提高，0.6%组的到显著差异（P<0.05）。丙二醛（MDA）含量随精氨酸添加量的提高成下降趋势。综上所述，夏季日粮中添加精氨酸可提高公猪精液抗氧化能力。30660
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毕业论文关键词：高温；精氨酸；抗氧化能力
Effect of Arginine on antioxidant capacity of boar semen during high temperature season
Abstract:The experiment added Arginine in summer so that explored the effect of Arginine on antioxidant capacity of boar semen under high temperature season.20 adult boars of the same breed (12-24 months old) who had similar weight and semen quality were selected.During the 2 month feeding period,they were fed with refined feed containing Arginine mass fraction of 0%,0.6%, 0.8% and 1% respectively.Then collected semen and calibrated the activity of Catalase (CAT), Glutathione Peroxidase (GSH-pH) and Total Superoxide Dismutase (T-SOD) and the content of Malondialdehyde (MDA) in seminal plasma.The results indicated that adding Arginine in summer can increase the activity of Catalase (CAT) in boar semen,and the 1%group was higher than that in the control group, and the difference was significant (P<0.05).In addition,dietary Arginine level had a positive effect on the activity of Glutathione Peroxidase (GSH-pX),and the 0.8% group and the 1.0% group had notable difference as the control group (P<0.05).Meanwhile,the activity of Total Superoxide Dismutase (T-SOD) was improved, and the 0.6%group showed a marked difference (P<0.05).On the contrary,the content of Malondialdehyde (MDA) decreased with the increase of Arginine.To sum up, the supplementation of Arginine in summer diet could promote the antioxidant capacity of boar semen.
Key words: High temperature;Arginine;Antioxidant capacity
目 录
摘要1
关键词1
Abstract…1
Key words1
引言…1
1材料与方法…2
1.1试验日粮 …2
1.2试验设计 …3
1.3样品采集与制备 3
1.4测定指标及操作步骤 3
1.4.1过氧化氢酶的活性 3
1.4.2谷胱甘肽过氧化物酶的活性 4
1.4.3总超氧化物歧化酶的活性 …4
1.4.4丙二醛的含量 5
1.5数据处理 …5
2结果与分析…5
2.1日粮精氨酸水平对高温季节公猪精液过氧化氢酶活性的影响…5
2.2日粮精氨酸水平对高温季节公猪精液谷胱甘肽过氧化物酶活性的影响…6
2.3日粮精氨酸水平对高温季节公猪精液总超氧化物歧化酶活性的影响6
2.4日粮精氨酸水平对高温季节公猪精液丙二醛的含量的影响6
3讨论7
3.1日粮精氨酸水平对高温季节公猪精液过氧化氢酶活性的影响…7
3.2日粮精氨酸水平对高温季节公猪精液谷胱甘肽过氧化物酶活性的影响…7
3.3日粮精氨酸水平对高温季节公猪精液总超氧化物歧化酶活性的影响7
3.4日粮精氨酸水平对高温季节公猪精液丙二醛的含量的影响7
4结论8
致谢…8
参考文献…8