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摘 要：以蚕豆种子为实验材料，采用了水培的方法，研究了不同浓度（0、20、25、30、35、40mg•L-1）SA对NaHCO3胁迫下蚕豆幼苗光合特性和抗氧化系统的影响，以期为利用SA缓解蚕豆生产过程中的土壤盐碱化伤害提供一定理论依据和技术支撑。经实验验证，蚕豆在NaHCO3 胁迫下，它的根系活力降低，便会影响根系的正常功能，从而对植物的光合特性和抗氧化系统造成影响。可以从多次实验得到，外施水杨酸显著提高了NaHCO3胁迫下光合速率，抗氧化酶的活性和根系活力，膜脂中的过氧化产物 MDA的含量也明显降低。外源SA可以文持细胞膜的稳定性，降低膜脂中过氧化物对细胞膜的伤害，在一定程度上提高植物的抗碱性。29893
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毕业论文关键字：外源SA；NaHCO3胁迫；蚕豆幼苗；光合特性；抗氧化系统
The Influence of Exogenous SA to Broad Bean Seedling Photosynthetic Characteristics and Antioxidant System under NaHCO3 Stress
Abstract: With broad bean seeds as experimental material , adopted a hydroponic method , studied the influence of the different concentration (0 , 20 , 25 , 30 , 35 and 40 mg/L) SA to broad bean seedling photosynthetic characteristics and the antioxidant system under NaHCO3 stress , in order to use the SA alleviate soil salinization damage in the process of broad bean production provide a certain theoretical basis and technical support . Under NaHCO3 stress , plants on the absorption of ions is suppressed , ion balance of plant cells is destroyed , which affects the root physiological function , the root structure is destroyed , and even lead to a big impact on the growth of plants(such as: the photosynthetic characteristics and antioxidant system) . By the experimental results can be obtained , applied salicylic acid significantly increased the photosynthetic rate , the activity of antioxidant enzymes and root work force increased significantly , also make the membrane lipid peroxidation products MDA content significantly decreased under NaHCO3 stress(P < 0.01) . Thus it can be seen that exogenous SA can be able to maintain the stability of cell membrane , reduce the membrane lipid peroxide damage to cell membrane , to a certain extent , improve the alkali resistance of plants .
Key words : Exogenous SA ; NaHCO3 stress ; Broad bean seedling ; The photosynthetic characteristics ; Antioxidant system
目 录
摘 要 1
Abstract 1
引言 2
1. 材料与方法 3
1.1 实验材料 3
1.2 处理方法 3
1.3 测定项目与方法 3
1.4 数据分析 4
2. 结果与分析 4
2.1 外源SA对NaHCO3胁迫下蚕豆幼苗光合色素的影响 4
2.2 外源SA对NaHCO3胁迫下蚕豆幼苗根系活力的影响 5
2.3 外源SA对NaHCO3胁迫下蚕豆幼苗超氧阴离子产生速率的影响 6
2.4 外源SA对NaHCO3胁迫下蚕豆幼苗SOD活性的影响 6
2.5 外源SA对NaHCO3胁迫下蚕豆幼苗POD活性的影响 7
2.6 外源SA对NaHCO3胁迫下蚕豆幼苗CAT活性的影响 8
2.7 外源SA对NaHCO3胁迫下蚕豆幼苗MDA含量的影响 9
3. 讨论 10
参考文献 12
致谢 15
外源SA对NaHCO3胁迫下蚕豆幼苗光合特性和抗氧化系统的影响
引言
蚕豆（Vicia faba L.），一年或两年生草本植物[1]。蚕豆主要分布于亚洲中西部[2]，是我国形体最大的食用豆类[3]。我国是蚕豆种植大国，我国的的蚕豆种植面积占世界蚕豆种植面积的61%。蚕豆营养价值很高，具有粮食、饲料、肥料和蔬菜、药用等多种用途，因此被广泛栽种[5]。