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摘要：油菜的杂种优势在油菜育种与种植方面具有重要意义，具体表现在生长快、抗逆性强和产量高等方面。但目前尚不清楚杂种油菜抗盐胁迫的生理性优势，因此本次实验着重于研究甘蓝型油菜杂种与亲本在盐胁迫下的生理优势比较。本实验以甘蓝型油菜品种J4375与CP3508，以及以这两个品种作为亲本杂交产生的F1代杂交种为材料，观察三个品系油菜在盐胁迫（200 mM NaCl）下的生长表现，对三个品系油菜的生理生化指标进行对比，并探究其耐盐性原理。实验研究发现，与亲本相比，杂交品系的脯氨酸（Proline, Pro）含量显著升高，一氧化氮(Nitric oxide, NO)水平也有所提升，并且，杂交种的叶绿素（Chlorophyll）含量、K+/Na+和相对含水量（Relative water content, RWC）也显著高于亲本品系。除此之外，实验发现，杂交品系的丙二醛（Malondialdehyde, MDA）含量比亲本低。以上实验结果说明杂交品系油菜在盐胁迫下的生理代谢比亲本更具有优势。本实验为油菜耐盐胁迫提供了一个新的思路。25915
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毕业论文关键词：甘蓝型油菜；杂种优势；盐胁迫；生理指标
Comparison of Physiological Advantages of Hybrid and Parental Brassica napus L. under Salt Stress
Abstract：The heterosis of rapeseed is of great significance in rape breeding and planting, showing in faster growing, stronger resistance and higher yield, etc. However, the physiological advantages of hybrid rapeseeds tolerance to salt (NaCl) stress are still not so clear, so this project focused on the comparisons of physiological advantages under salt (NaCl) stress between Brassica napus hybrids and their parents. In this research, the F1 hybrids of Brassica napus L. J4375 and CP3508, and parents themselves were used as the materials to observe the growth performance of three lines of rapeseed under salt stress (200 mM NaCl). The physiological and biochemical indexes of rapeseed were compared to explore the salt tolerance principles. In this study, it was found that the proline (Pro) content of hybrids was significantly more than that of the parents, and the level of nitric oxide (NO) was also increased. The chlorophyll content, K+/Na+ and relative water content (RWC) of the hybrid were higher compared to those of the parents. In addition, it was found that the content of malondialdehyde (MDA) in the hybrids was not as much as that of the parent. Results above showed that the physiological metabolism of rapeseed under salt stress is more advantageous than that of the parents. In this project, the physiological advantages of hybrids in salt tolerance were found by experiments, which might provide a new idea for salt tolerance of rapeseeds.
Key words: Brassica napus L; Heterisis; Salt stress; Physiological indicators
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1.1 材料 2
1.2 方法 2
1.2.1 实验设计2
1.2.2激光共聚焦显微镜测量植物根细胞中一氧化氮（Nitric oxide, NO）含量3
1.2.3 TBA法测定植物中丙二醛（Malondialdehyde, MDA）含量3
1.2.4酸性茚三酮测定脯氨酸（Proline, Pro）含量3
1.2.5 叶绿素（Chlorophyll）含量测定3
1.2.6 植物组织干鲜重比（Relative water content, RWC）及K+/Na+测定3
1.2.7 Greiss试剂法测定植物叶片组织中的一氧化氮含量4
1.2.8 数据分析处理4
2 结果与分析4
2.1 盐胁迫对三品系油菜K+/Na+的影响4
2.2盐胁迫对三品系油菜相对含水量（RWC）的影响5
2.3盐胁迫对三品系油菜叶片叶绿素含量的影响6
2.4盐胁迫对三品系油菜叶片脯氨酸含量的影响7
2.5盐胁迫对三品系油菜的NO含量的影响7
2.6盐胁迫对三品系油菜的丙二醛（MDA）含量的影响8
2.7盐胁迫下三品系油菜根部细胞中的NO含量8