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摘要：氯噻啉是一种作用于烟酸乙酰胆碱酯酶受体的新烟碱类内吸性杀虫剂，广泛应用于防治刺吸式口器害虫，具有高效、广谱、低毒等优点。随着氯噻啉用量日渐增加，对其在农产品和环境中残留检测方法的研究也已广泛开展。其中，免疫检测分析技术在农药残留检测中的发展较快，应用也逐渐广泛起来。它具有灵敏、快速、特异性强、分析容量大、安全可靠等优点。免疫磁珠分离技术是将免疫学反应的高度特异性与磁珠特有的富集特性相结合的一种新颖的免疫学检测技术。Fe3O4磁性纳米材料（磁珠，MNPs）和上转换荧光纳米材料（UCNPs）在分离富集效应和光学性质上有各自的优势，MNPs和UCNPs组装起来形成的复合纳米材料将同时具备两者的优势。本研究以氯噻啉农药为研究对象，首先合成出MNPs与UCNPs，基于磁分离原理，建立了氯噻啉上转换荧光免疫分析方法，并应用于农产品和环境样品中氯噻啉残留的检测分析。25562
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毕业论文关键词：氯噻啉；免疫磁珠分离；荧光纳米材料；上转换荧光免疫分析
The Development of Upconversion Fluorescence Immunoassay Based on Magnetic Beads
Abstract: Imidaclothiz is a kind of new neonicotinoid systemic insecticide acting on Nicotinic acetylcholine receptor.It has the advantages of high efficiency, low toxicity, broad spectrum. With the increasing of the use, the study on the methods of detecting residues in agricultural products and environment has been carried out extensively. The development of immunoassay of pesticide residues detection is rapid, the application is also gradually widely. It is sensitive, rapid, specific, large capacity, safe and reliable. Immunomagnetic bead isolation technology is a new immunoassay technology which combines the high specificity of the immunological reaction with the enrichment characteristics of magnetic beads. Assembled together to form composite nanomaterials, Fe3O4 magnetic nanoparticles and upconversion fluorescent nanomaterials play their respective advantages in separation and enrichment effect and optical characteristics. In the study, composite nanomaterials of Fe3O4 magnetic nanoparticles and upconversion fluorescent nanomaterials are synthesized with magnetic separation technology. The upconversion fluorescence immunoassay method is established and applied to analysis of the residues of Imidaclothiz in produce and environmental samples.
Key words: Imidaclothiz; Immunomagnetic separation; Fluorescent nanomaterials; Upconversion Fluorescence Immunoassay
目 录
引言 2
1 材料与方法 3
1.1 材料 3
1.1.1 主要试剂 3
1.1.2 主要仪器 3
1.2 方法 4
1.2.1 上转换纳米材料（UCNPs）合成 4
1.2.2 上转换纳米材料（UCNPs）氨基功能化 4
1.2.3 氨基功能化Fe3O4 磁性纳米颗粒（MNPs）合成 4
1.2.4 纳米材料与氯噻啉人工抗体、抗原偶联 4
1.2.5 基于UCNPs和MNPs免疫分析 4
1.2.6 免疫分析工作条件优化 5
1.2.7 选择性评价（交叉反应） 5
1.2.8 添加回收实验 5
1.2.9 准确性验证 5
2 结果与分析 6
2.1 磁性纳米颗粒表征 6
2.2 免疫分析工作条件优化 7
2.3 灵敏度分析 8